Choosing the right immersion oil
For Normal Light Microscopy: Types A and B are virtually interchangeable and are miscible with each other for intermediate viscosities. Produced in larger quantities than other types, Types A and B are the most economical. The deciding factor in choosing between them is the optimum viscosity for your particular application. Type A, at 150 centistokes, reduces any tendency to trap air, especially helpful to beginning students. Air bubbles cause image degradation. Type B, at 1250 cSt, is thick enough for viewing multiple slides with one application. This saves time during batch processing.
Automated
Hematology Systems: Use Type 300; Automated Hematology Systems depend
on accurate, precisely controlled physical and optical properties of
immersion oil for successful imaging and mechanical processing.
Type 300 is designed and manufactured to meet the stringent
requirements of this equipment, which include specialized viscosity and
exacting controls for its consistency. Inverted, Inclined,
Projection, and Long Focus Instruments:
Use type NVH or OVH; The greater the gap between the cover glass and
objective, or between the slide and condenser, the more desirable high
viscosity becomes. The very high viscosities of Type NVH at 21,000 cSt and Type OVH at 46,000 cSt give excellent results for these applications.
Blending
Oils from the Miscible Group: The Miscible Group of immersion oils is
A, B, 300, NVH and OVH. Users can easily blend any two immersion oils
from the Miscible Group to obtain an immersion oil with an intermediate
viscosity while maintaining the optical properties common to both.
Fluorescence
Microscopy: Extremely low fluorescence is achieved by Type DF and Type
HF. Type FF is virtually fluorescence-free, though not ISO compliant.
Type HF is slightly more fluorescent than Type DF, but is halogen-free.
The Best Little Bottle!
Does it drive you nuts that immersion oil only seems to come in 1/4 ounce sizes? Are you constantly opening a new bottle because of the amount of oil in the bottle? Back when Fryer Company (Huntley, IL) was the largest Nikon Dealer in the world, they worked with labs to come up with the perfect size, 1.25 oz, and shaped applicator tip to make it easier to use. When Fryer Company closed many of its customers thought the bottle was gone. Blue Marble Products has acquired the bottle and has now made it available to all customers. Check out the bottle and our prices. We look forward to hearing from you soon!